OriCiro Launches World's First Cell-Free Amplification Technology for Large Circular DNA

TOKYO, Sept.A 30, 2020 /PRNewswire/ -- OriCiro Genomics, a pioneer in the cell-free synthesis and amplification of genome-scale large DNA for gene therapy and synthetic biology, has today launched the OriCiroTM Cell-Free Cloning System, the world's first technology enabling cell-free amplification of large circular DNA, eliminating the reliance on E. coli cloning.Currently, researchers in life sciences use cell-based methods to clone large DNA molecules. Although these approaches remain the gold standard, they are infamous for being time-consuming, inefficient, and unable to deal with cell-toxic DNA sequences. The OriCiroTM Cell-Free Cloning System solves all these problems.The OriCiroTM Cell-Free Cloning System comprises the OriCiro TM Assembly Kit and OriCiro TM Amp Kit. A simple two-step in vitroA process enables cell-free assembly and amplification of circular DNA molecules without the need for E. coli transformation and culture.

OriCiro TM Assembly

A simple one-step process enables the seamless assembly of multiple DNA fragments via homologous overlapping ends (40bp). A unique enzymatic annealing feature enables simultaneous assembly of up to 50 fragments in just 30 minutes.

OriCiro TM Amp

The kit contains a mixture of 26 purified proteins that reconstitutes the E. coli genome propagation process in vitro, exhibiting outstanding performance in amplifying large DNA molecules, up to 1 Mbp in size, with extremely high fidelity. Circularized assembled molecules from the assembly reaction are selectively amplified during several hours of isothermal incubation to yield a high concentration of pure, super-coiled circular DNA.
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